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Antioxidant leaf thesis

antioxidant leaf thesis

EG3 showed the biggest change in flavonoid concentrations in response to the stress, but the Egyptian lines (especially the non-tolerant EG lines) in general were most responsive to the stress, whereas the tEU and tAF were the least responsive. Its accumulation during stress initiates the response of the plant defense system, including the accumulation of antioxidants and activation of redox-regulating enzymes. A homogenate of the leaves (1 g / 1 ml) was prepared in double distilled water, centrifuged at 2000 rpm for 5 minutes and the supernatant was used as the fresh aqueous extract. The study present the first report on the scavenging activity. Several epidemiological studies suggest that plants rich in antioxidants play a protective role in health and against diseases, and their consumption lowered the risk of cancer, heart disease, hypertension and stroke5. Received ; Revised ; Accepted ; Published 16 November 2014.

(PDF antioxidant activity of leaf different extracts in Punica granatum

Cytotoxic effect and anti-inflammatory activity. Screening for drought tolerance of antioxidant leaf thesis maize hybrids by multi-scale analysis of root and shoot traits at the seedling stage. Doi:.1093/jxb/ers277 CrossRef Full Text Google Scholar Xiong,., and Zhu,. The fluorogenic cell permeant, H2DCF-DA (green cells was used to measure the level of reactive oxygen species (ROS) in activated Raw 264.7 macrophage cells induced by LPS and PMA stimulants (Figure 4 ). Therefore, we conclude that the MDA contents across the growth zone of drought tolerant lines were somewhat higher in control conditions, but changed less in response to drought. The concentration of oxidative stress determinants, antioxidant molecules, and enzyme activities. Initiation and inhibition of free-radical processes in biochemical peroxide systems: a review. The anova analysis showed significant differences for 7 factors and between-factor interactions, demonstrating that the origin of the lines (significant O) is one of the main factors determining global differences in total antioxidant capacity as well as the response. Antioxidant levels were approximately stable across the growth zone and increased proportionally with the drought levels (Figure 2C ). Chem Biol Interact 2006; 160: 1-40. This could be related to the fact that, next to the damaging effect of H2O2 accumulation in the leaf, basal H2O2 concentrations promote cell elongation by mediating cell wall loosening shown in roots ( Liszkay. On the other hand, tEU1 and EG3 clustered separately from the rest, having a more pronounced response to the stress than the other lines. Lipid peroxidation assessment by malondialdehyde measurement in parenteral nutrition solutions for newborn infants: a pilot study.

The ferric reducing ability of plasma (frap) as a measure of antioxidant power: the frap assay. It entails leaf-elongation rate and final leaf length measurements, measurements of the cell-length profile along the axis of the leaf, and estimation of the size of the leaf basal meristem by locating mitotic cells in dapi-stained leaf sections. Methanol extract had LC50 of 784 g/mL after 24-hour exposure, hexane had 629.3 g/mL and dichloromethane 573.6 g/mL, and 544.6 g/mL was the lowest with acetone extract. Secondary metabolites are responsible for biological activities of plants including terpenoids 11, phenolic compounds (flavonoids, phenolic acids, quinones, coumarins, lignans, stilbenes, tannins and nitrogen compounds (alkaloids, amines, and betalains) and carotenoids. Drought tolerance closely correlated with the MDA concentration in the growth zone (Table S2 and Figure 2A MDA accumulated particularly in the mature part of the leaves of the sensitive lines in response to the stress showing that these lines experienced more lipid peroxidation. Effects of salinity on the transcriptome of growing maize leaf cells point at cell-age specificity in the involvement of the antioxidative response in cell growth restriction. M., and Chern,.

Antioxidant effects of Artemisia vulgaris leaf extracts

Nelissen., 2012 ). Doi:.1007/s CrossRef Full Text Google Scholar ipcc (2014). This could be related to increased apoplastic antioxidant enzyme levels in the apical region ( Zhu., 2007 ) and elevated H2O2 levels ( Voothuluru and Sharp, 2013 suggesting that the apical (meristematic) region may be protected from ROS. Consistently, the most sensitive lines (the 3 EG hybrids) clustered together for almost all the measured parameters. A decrease in catalase activity was found in H2O2-exposed liver slices when compared to the control group (Figure 2). The depletion of GST with the exposure of H2O2 was counteracted by the co-administration of leaf extracts. Figure 6: Effect of Artemisia vulgaris leaf extracts on vitamin C level.

Investigation of antioxidant and antimicrobial activities

Antioxidant activity of all crude extracts was quantitatively measured against 2,2-azino-bis acid) free radical molecules using abts assay. LC50 indicates the lowest concentration of plant extracts that inhibits 50 of cells. The details of the protocol are described by Avramova. In the Egyptian hybrids and B73 the opposite pattern occurred: ASC concentrations were the highest in the severe drought conditions. Communis using other plants parts. Nguyen., 2004 ). S., Plumbdhindsa,., and Thorpe,. Hundred microliters of cells (4 104 cells/mL) was seeded in 96-well plates and incubated at 37C, 5 carbon dioxide for 24 hours. The liver slices were treated in Page. Yield potential, yield stability and stress tolerance in maize. Methanol extract had the highest percentage free radical (abts) scavenging activity of 95.50 mg/mL, acetone 91, dichloromethane 62, and hexane the least (50). Results can be calculated using the differences in areas under the phycoerythrin decay curves between the blank and a sample and are expressed in Trolox equivalents. Physiology and molecular biology of salinity stress tolerance in plants.

In Vitro Assessment of Cytotoxicity, Antioxidant, and, anti -Inflammatory Liu J, Gu X, Robbins D, Li G, Shi R, McCord JM,. After 45 min incubation, the Fe3-xylenol orange complex was measured at 595. Leaf elongation rates ( LER ) of all the lines were reduced more than LL, demonstrating that compensation by increased duration of the leaf growth occurs. Our biochemical measurements showed a clear antioxidant leaf thesis separation between the Egyptian and other hybrids according to most of the measured parameters (ASC, GSH, tASC, tGSH, total antioxidant capacity, polyphenols, flavonoids, SOD, CAT, POX, dhar, mdar). Redox Status and Antioxidant Content in the Maize Leaf Next, we set out to investigate if differences in stress defenses in the leaf growth zone could be related to the differences in cellular responses to drought.

Reactive oxygen species in the elongation zone of maize leaves are necessary for leaf extension. In control conditions, the tEU and tEG hybrids had the highest MDA levels in their growth zones, and the EG hybrids and B73 the lowest (Table 3 ). Previously, we showed the changes of the H2O2 gradient in B73 in response to our antioxidant leaf thesis drought treatments, and linked these to the mechanisms of leaf growth reduction ( Avramova., 2015a ). Iol 2006; 329: 775-784. Half of the European and the African hybrids were rated as drought tolerant (see Avramova., 2016 ). Conspicuously, there were no interaction effects with location in the growth zone (interactions with L) indicating that, although H2O2 levels vary along the growth zone (significant L, Figure 2B they are affected proportionally by drought and genotype across the growth zone. Cells were fixed and stained with H2DCF-DA to measure the amount of free radical molecules produced in the cells (green) and dapi to locate the nucleus in the cells (blue). The safety of plants as a potential therapeutically agents must be ascertained and the side effects should be acceptable to the host. The outcome of the present study showed that the leaves. Figure 2: Percentage scavenging activity of abts free radicals with respect to increasing concentration. Non-Enzymic Antioxidant Levels The non-enzymic antioxidants, namely vitamins C, E, A and reduced glutathione were estimated in the oxidant challenged liver slices with or without the leaf extracts. The root extracts.

Activity Facilitates Maintenance of Cell

Doi:.1104/pp.107.107250 PubMed Abstract CrossRef Full Text Google Scholar. Methanol extracts. Extracellular H2O2 induced by oligogalacturonides is not involved in the inhibition of the auxin-regulated rolB gene expression in tobacco leaf explants. In terms of drought tolerance, tAF and tEU had higher polyphenol levels (0.43 and.39 mg GA/g antioxidant leaf thesis FW, respectively) in control conditions than AF and EU (0.39 and.38 mg GA/g FW, respectively but the opposite was. The possibility that active constituent present in the roots may be responsible for observed activity in the leaves may not be ruled out.

Antioxidant - an overview ScienceDirect Topics

Quantitative Antioxidant Assay Using abts A stock solution of 7 mM of abts was prepared according to van den Berg. Acknowledgment The authors would like to acknowledge National Research Foundation (NRF) for financial support. Communis was also antioxidant leaf thesis quantified using abts decolonization method. Principal component analysis of variation in antioxidant metabolite levels and enzyme activities across maize lines in response to drought. The differences in the response to the drought conditions between hybrids with different drought tolerance in the field (based on breeder's ranking different origin and between hybrids and the inbred line at the early seedling stage, allowed. Precision-cut liver slices can contribute to the reduction of animal experiments.